INVESTIGADORES
OTERO Maria Claudia
artículos
Título:
Vaginal bacterial diversity from healthy gilts and pregnant sows subjected to natural mating or artificial insemination
Autor/es:
TORRES LUQUE, A.; FONTANA, C.; PASTERIS, S.E.; BASSI, D.; COCCONCELLI, P.S.; OTERO, M. C.
Revista:
RESEARCH IN VETERINARY SCIENCE
Editorial:
ELSEVIER SCI LTD
Referencias:
Lugar: Amsterdam; Año: 2021 vol. 140 p. 26 - 37
ISSN:
0034-5288
Resumen:
The profitability of commercial pig farms largely depends on the reproductive performance of gilts and sows. The aim of this study was to identify differences in the composition and diversity of vaginal microbiota between gilts (G) and pregnant (P) sows, both artificially inseminated (AI) and natural mating (NM). The samples were collected by scraping the vaginal mucosa of G (n = 10) and P (NM, n = 10 and AI, n = 7) sows. Samples were analysed by culture-dependent techniques and 16S-rRNA gene High-Throughput-Sequencing. The profiles of the cultured microbiota showed two distinctive clusters, one of them grouped four samples of P sows from the AI group. The vaginal microbiota from P had lower richness than G sows (Mann-Whitney/Kruskal-Wallis test, p < 0.01), but all vaginal samples had a similar diversity. The PERMANOVA analyses revealed significant differences (p < 0.01) between the microbial communities? structures from G, NM and AI sows. The bacteria phyla with the highest relative abundances were Proteobacteria (33.1%), followed by Firmicutes (32%), Cyanobacteria (13.3%) and Actinobacteria (13.2%). The relative abundance for phyla, families and genera was estimated and Proteobacteria was significantly higher (p = 0.038) in P than in G sows; Firmicutes was significantly lower in AI than G and NM sows. A ?core microbiota? included Lactobacillus, Bacillus, Enterococcus, Acinetobacter and Pseudomonas. The results presented highlight the differences in the vaginal bacterial composition between two groups of healthy females, G and P sows, as well as the changes in the microbial populations associated with the breeding method.