In vitro assessment of a resorbable scaffold for skin tissue engineering

GONZALEZ WUSENER AE; RUIZ IE; ARREGUI CO; BOLGIANI AN; HERMIDA E

Congreso; EMBO Conference on The molecular and cellular basis of regeneration and tissue repair; 2016

PurposeOne of the major applications of tissue-engineered skin scaffolds is to promote the healing ofacute and chronic wounds. Once set onto the wound bed and seeded with keratinocytes, skinscaffolds act as a template for dermal and epidermal formation. Among the aspects mediatinghealing, the main mechanisms of action are: (i) colonization of the scaffold by fibroblasts, (ii)provision of a suitable substrate for the attachment and proliferation of keratinocytes, and (iii)transfer of growth factors from the fibroblasts of the wound bed to enhance keratinocytesproliferation and differentiation, without direct interaction of the two cell types. The aim of thiswork is to assess: the cytotoxicity of the scaffolds, the migration of fibroblasts through thescaffold, and the proliferation of keratinocytes seeded onto the scaffolds.MethodsIn vitro assessments were performed in PHBV scaffolds developed and optimized at our lab.Cytotoxicity tests were performed according to the ISO 10993-5 standard. Adhesion andproliferation assays were performed with murine and human cells, analyzed with fluorescencemicroscopy and quantitative methods.ResultsResorbable membranes did not induce cytotoxic effects on cells in culture. Moreover, the scaffoldspromoted adhesion and proliferation of keratinocytes in vitro, as well as fibroblasts.ConclusionThe 3D porous scaffolds produced in our lab achieve the main features of scaffolds suitable forskin regeneration.