INVESTIGADORES
GONZALEZ SANCHEZ WUSENER Ana Elena
congresos y reuniones científicas
Título:
In Vitro Assessment of Resorbable Scaffold for Skin Tissue Engineering
Autor/es:
ANA GONZALEZ WUSENER; IGNACIO E RUIZ; CARLOS O. ARREGUI; ALBERTO N. BOLGIANI; ÉLIDA B. HERMIDA
Reunión:
Congreso; Tissue Engineering & Regenerative Medicine International Society Americas Annual Meeting; 2014
Resumen:
One of themajor applications of tissue-engineered skin scaffolds isto promote the healing of acute and chronic wounds. Once set onto the wound bed and seeded with keratinocytes, skin scaffolds act as a template for dermal and epidermal formation. Among the aspects mediating healing,the main mechanisms of action are: (i) colonization of the scaffold by live fibroblasts, (ii) provision of a suitable substrate for the attachment and proliferation ofkeratinocytes, and (iii) transfer of growth factorsfrom the fibroblasts of the wound bedto enhance keratinocytes proliferation and differentiation, without direct interaction of the two cell types. The aim of this work is to assess:the cytotoxicity of the scaffolds, the migration of fibroblasts through the scaffold, and the proliferation of keratinocytes seeded onto the scaffolds. The conclusion is the feasibility of 3D porous scaffolds produced in our lab to achieve the main features of scaffolds suitable for skin regeneration. Acknowledgements: This work has been partially supported by FONARSEC, Project EMPRETECNO Nº 37, the National University of San Martín (UNSAM) and the National Council of Scientific and Technical Research (CONICET). Disclosures:The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in, or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.