Purification of the Lipase B of Candida Antarctica from a Commercial Enzymatic Extract

C. R. LLERENA SUSTER; L. E. BRIAND; S. R. MORCELLE

Current Catalysis

Bentham Science

Año: 2014 vol. 3 p. 155 - 160

2211-5447

This paper presents a rational strategy to purify the lipase B of Candida antarctica of the commercial extract Lipozyme® through size exclusion coupled with anionic exchange chromatography using a non conventional, easy to remove buffer system such as ammonia-ammonium. In this context, each step of the purification was followed through the determination of the protein content, esterase activity measurements, SDS-PAGE, agarose electrophoresis, UV-spectroscopy and isoelectric focusing. The purification of the commercial extract afforded a sample that retains 47% of the proteins (being CALB the major enzymatic component of the purified sample) with a hydrolytic activity higher than the starting crude extract.