SLPI (Secretory leukocyte protease inhibitor) induces apoptosis on mammary tumor cells.

AMIANO, NICOLÁS; COSTA, JULIETA; ROMEO, DOLORES; ORQUEDA, ANDRÉS; SILVIA ESTEIN; CHULUYAN, EDUARDO

Congreso; First French-Argentine Immunology Congress; 2010

SLPI is an 11.7 kDa protein, characterized as an anti-inflammatory agent. It has been described that SLPI is expressed in several tumor cell lines. However, the role of SLPI in cancer is not completely elucidated. Previously studies in our laboratory showed that SLPI over-expressing cells do not develop tumor in mice. The aim of this work was to analyze the ability of SLPI to induce apoptosis of mammary tumor cells. Apoptosis was study on murine F3II cell line (mammary carcinoma) and 2C1 (F3II derived cells over-expressing SLPI). Apoptosis of 2C1 and F3II cells was measured by flow cytometry with FITC-Annexin V Kit and microscopy by ethidium bromide/acridine orange staining. The apoptosis of 2C1 cells was significantly higher than F3II control cells (45 ± 5% vs 16 ± 3%, p