INVESTIGADORES
CHULUYAN Hector Eduardo
congresos y reuniones científicas
Título:
Slpi modulates the expression of adhesion and histocompatibility molecules on monocytes and hela cells.
Autor/es:
NICOLÁS AMIANO, DIEGO GUERRIERI, JULIETA COSTA, PAULO MAFFIA, EDUARDO CHULUYAN
Lugar:
Cordoba, Argentina
Reunión:
Congreso; 7º congreso de la Asociacion Latinoamericana de Inmunologia; 2005
Institución organizadora:
ALAI
Resumen:
AbstractThe aim of this work was to study the ability of Secretory Leucocyte Protease Inhibitor (SLPI) to modulate the expression of molecules on monocytes and HeLa. The expression of CD54, MHC I and MHC II were analyzed with flow cytometry on cells. The treatment of monocytes with rhSLPI (4mg/ml, 16h, 37°C) decreased the expression of CD54(22%) and MHC II(9%), but not MHC I. The expression of CD54 was also decreased (30%) on HeLa cell line after the treatment. Furthermore, unlike for monocytes, the expression of MHC I was also decreased (40%) on HeLa cells. Since, HeLa produced significant amount of SLPI, we examined whether endogenous SLPI was able to modulate the expression of MHC I and CD54. By transfected HeLa with SLPI antisense oligonucleotides, the production of SLPI was reduced by 16% and the expression of MHC I increased by 27%. To improve the downmodulation of SLPI, the gene of SLPI was cloned with antisense orientation into mammalian expression vector (pcDNA3). Following, HeLa was transfected with this construction. Similar to the results obtained with antisense oligonucleotides, the expression of SLPI on the stable transfected cell line, was lower than non transfected cells and the expression of MHC I increased significantly (30%). Overall, these results suggest that endogenous and exogenous SLPI can modulate the expression of cell surface molecules.