INVESTIGADORES
CHULUYAN Hector Eduardo
congresos y reuniones científicas
Título:
PRE-ELAFIN: a Th2 cytokine profile inducer?
Autor/es:
29. TATEOSIAN, NANCY; GUERRIERI, DIEGO; ALIJA, ANA; GARCIA, VERÓNICA; CHULUYAN, EDUARDO.
Lugar:
Buenos Aires
Reunión:
Congreso; First French-Argentine Immunology Congress; 2010
Institución organizadora:
SAI
Resumen:
PRE-ELAFIN (skin derived anti-leukoproteinase) is a 9.9kDa serineproteinase inhibitor, precursor of ELAFIN which containsmultiple transglutaminase substrate domains. The increasedPRE-ELAFIN expression is well documented in inflammatoryconditions in epidermis, bronchial epithelial cells, intestinal epitheliumand endometrial epithelial cells. The aim of the presentwork was to investigate whether PRE-ELAFIN modifies the Th1or Th2 cell pattern of cytokines. First, peripheral blood mononuclearcells (PBMC) were incubated with IL-2 in the presence ornot of rhPRE-ELAFIN. After 5 days, proliferation was measuredby [3H]-Thymidine uptake. We observed that rhPRE-ELAFINdecreased lymphocyte proliferation induced by IL-2 (control:76.4±20.6% vs 20.3±2.3%, p<0.05). This effect was dose-dependent.Furthermore, cytokines were measured in the cell culturesupernatant with a flow cytometry bead array kit. An increasedof IL-4, IL-6 and IL-10 was observed in the culture supernatantof PBMC treated with rhPRE-ELAFIN (IL-4 control: 147±28 pg/ml vs 948±328 pg/ml, p<0.05; IL-6 control: 2370±1024 pg/ml vs4475±1020 pg/ml, p<0.05; IL-10 control: 99±41 pg/ml vs 430±155pg/ml, p<0.05), while it did not modify IFNγ levels. The increaseproduction of IL-4 by PBMC treated with rhPRE-ELAFIN was confirmedby performing an intracellular staining of IL-4 on T cells.Overall, these results suggest that PRE-ELAFIN is a Th2 factorinducer.