INVESTIGADORES
MARRASSINI Carla
congresos y reuniones científicas
Título:
COMPARATIVE STUDY OF THE ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF TWO SPECIES OF Urera aurantiaca FROM DIFFERENT GEOGRAPHICAL AREAS: THEIR RELATION TO POLYPHENOLS CONTENT
Autor/es:
MARRASSINI CARLA; PERALTA IGNACIO; ANESINI CLAUDIA
Lugar:
Buenos Aires
Reunión:
Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017
Institución organizadora:
SOCIEDAD ARGENTINA DE FARMACOLOGÍA EXPERIMENTAL (SAFE)
Resumen:
Urera auratiaca is an Argentinean species that has been traditionally used to treat symptoms of inflammation. The aim of this study was to determine comparatively the antioxidant and anti-inflammatory effects of two species of Urera aurantiaca coming from different geographical areas of Argentina: Salta (S) and Misiones (M). Methanol extracts were prepared to obtain polyphenolic compounds. Both extracts showed antioxidant activities and in vitro anti-inflammatory activities, results represent mean ± ESM of three experiments made in triplicate: DPPH radical scavenging activity S EC50: 245 ± 40 μg/ml, M EC50: 977 ± 20 μg/ml; Reducing power S EC50: 126 ± 3 μg/ml , M EC50: > 1000 ± 50 μg/ml; Inhibition of lipid peroxidation S EC50: 1862 ± 10 μg/ml, M EC50: 2000 ± 20 μg/ml; SOD like activity S EC50: 537 ± 37 μg/ml, M EC50: 363 ± 24 μg/ml; Inhibition of albumin denaturation S EC50: 812 ± 59 μg/ml, M EC50: > 1000 ± 25 μg/ml; Proteinase inhibitory action S EC50: 10 ± 2 μg/ml, M EC50: 42 ± 4 μg/ml. They also were able to reduce the augmented NO release in activated macrophages but S extract showed the largest inhibition: 76.6% at 500 μg/ml and was able to restore LPS to the basal levels. S extract showed the best antioxidant and anti-inflammatory properties in almost all the tested experiments probably due to the highest polyphenols content (S: 30.9 ± 1.8; M: 14.1± 2.6 GAE/g) and specifically due to the highest flavonoids content (S: 2.10 ± 0.02%; M: 1.49 ± 0.02%). These differences between the extracts are probably related to ambient diverse conditions: temperature, rain, soil composition and ecosystem.