SLPI improves the recellularization of renal scaffolds

HAEUBLEIN, GERALDINE; GUERRIERI DIEGO; AMBROSI, NELLA; CARO FIORELLA; SANCHEZ F; INCARDONA CLAUDIO; CASADEI DOMINGO; CHULUYAN EDUARDO

Congreso; Reunión Conjunta SAIC SAI SAFIS 2018; 2018

To deal with the problematic of the lack of donors for organ transplantation, regenerative medicine proposes the generation of histocompatible bioartificial organs by recellularizing scaffolds. In previous studies, we observed that secretory leukocyte protease inhibitor(SLPI) exhibits nephroprotective activity in vivo. The aim of this workwas to evaluate the use of SLPI to improve the recellularization of renal scaffolds with human proximal tubule epithelial(HK-2). First, weevaluated in vitro a protective effect of SLPI on HK-2 cell exposed toFK-506 by the MTT assay and annexin V/propidium iodide. Furthermore, we study the ability of SLPI to induce HK-2 proliferation andthe effect of SLPI in the wound healing assay. Finally, we producerat kidney scaffold with the constant admistration in renal artery of1% SDS (2ml/min) for 24 h followed by 24 h of 0.1% Triton X-100.After washing with saline solution, scaffolds were seeded throughthe ureter with 2.106 HK-2 cells previously treated or not with SLPI(4μg/ml). Our results showed that the incubation of HK-2 with SLPI(0,4 μg /ml) for 24 h reduce the cytotoxicity induced by FK-506 (p