INVESTIGADORES
GUERRIERI Diego
congresos y reuniones científicas
Título:
SLPI IS TAKEN UP BY MONOCYTES AND IMPAIRS THEIR DIFFERENTIATION TO DENDRITIC CELLS
Autor/es:
MARDIROSIAN M; CARO FIORELLA; AMBROSI NELLA; VILLALONGA XIMENA; MACARENA REITERI; GUERRIERI DIEGO; CASADEI DOMINGO; CHULUYAN EDUARDO
Reunión:
Congreso; Reunión Conjunta SAIC SAI SAFIS 2018; 2018
Resumen:
Secretory leukocyte proteinase inhibitor (SLPI) is a serine proteaseinhibitor produced mainly by epithelial cells. It has anti-inflammatoryand antimicrobial activity, and enhances wound healing. We havepreviously described that SLPI inhibits lymphocyte proliferationwhich depends on the presence of monocytes. The aim of the present study was to assess whether SLPI is captured by monocytesand its effect on monocytes differentiation to dendritic cells.To study SLPI uptake, human myelomonocytic U937 cells and human peripheral blood mononuclear cells (PBMC) were treated ornot with 10 µg/ml of rhSLPI for 1 h. Then, the cells were fixed andpermeabilized. Afterwards, the cells were incubated with an anti SLPI-FITC antibody and the fluorescence was analyzed by flow cytometry. To evaluate the effect of SLPI on monocytes differentiationto dendritic cells, human monocytes were isolated from PBMC andincubated with GM-CSF + IL-4 in the presence or absence of rhSLPIfor 5 days. The cells phenotype was characterized by flow cytometryusing anti-MHC I and II, CD86 and CD14 monoclonal antibodies.We observed that 45-50% of U937 cells cultured with SLPI but notwithout SLPI, were stained with SLPI-FITC but not with isotypecontrol monoclonal antibody. The same was observed for just only15-20 % of CD14+ human mononuclear cells. These results suggest that monocytes can incorporate SLPI from the medium. Moreover, as expected, monocytes treated with IL-4 + GM-CSF (5 days)showed high expression of CD86 and MHC II, intermediate expression of MHC I and very low expression of CD14. In contrast, whenmonocytes were incubated with IL-4 + GM-CSF in the presence ofSLPI, cells showed a high expression of CD14, low expression ofCD86 and MHC II.Overall, these results demonstrate that monocytes can incorporateSLPI from the microenvironment and suggest that SLPI alters theirdifferentiation to dendritic cells.