KIDNEY ACELLULAR MATRIX AND NEW STRATEGIES OF RECELLULARIZATION

GUERRIERI DIEGO; SANCHEZ F; HAENBLEIN G; TONIOLO FERNANDA; DRAGO J; ARANDA M; UVA PABLO; CASADEI DOMINGO; CHULUYAN EDUARDO

MAR DEL PLATA

Congreso; LXIV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA; 2016

Replacement therapy is the best treatment option against organ failure, however graft rejection and adverse effects of immunosuppression remain major clinical complications. The aim of this work was to obtain kidney acellular matrix and develop new strategies of recellularization. Wistar rats were used, which were made nephrectomy, then the kidney was cannulated for renal artery, placed in a bioreactor at room temperature and connected to a pump with a constant flow of 0.5 ml/min for the passage of 1% SDS and distilled water (18 h). Macroscopic changes in the kidney during the decellularization can be displayed in our video: (https://youtu.be/NI2OU-PE15g). In histological sections an acellular matrix was observed with preserved renal architecture being positive for markers of collagen and laminin. For recellularization process we develop an in vitro and another in vivo protocol. In the first, 1,5.106 human renal epithelial cells (HK-2) was administered by ureter. At 72 hours, with histological techniques viable cells mainly located in the cortical tubules were observed. For in vivo protocol, acellular scaffold were implanted into recipient rats in subcutaneous and intraperitoneal location for one or two weeks. Once recovered the scaffolds showed a large inflammatory infiltrate, along with some positive cells markers for transgenlin and podocin. Conclusion: These results show the feasibility of renal decellularization techniques for obtaining acellular scafolds, and the possibility of recellularize at least partially both in vitro and in vivo. However, the development of more complex recelullarization techniques is required to generate more complex and functional renal structures