Serine antiprotease trappin-2 induces IL-9 in human peripheral blood mononuclear cells

ALIJA ANA; AMBROSI NELLA; RODRIGO EMANUEL HERNÁNDEZ; GUERRIERI DIEGO; BELÉN ALVAREZ; TATEOSIAN NANCY; GARCIA VERONICA; CHULUYAN EDUARDO

hawai

Congreso; The american association of immunologists annual meeting 2013; 2013

 Trappin-2 is a 9.9 kDa serine protease inhibitor produced by epithelial cells and immune cells such as macrophages and γδT cells. It has anti-inflammatory and microbicide activities. The aim of the  resent work was to evaluate the effect of Trappin-2 on peripheral blood mononuclear cells (PBMC) by analyzing proliferation response, apoptosis, cytokine production and transcription factors expression. After 5 days of culture, Trappin-2 decreased lymphocyte proliferation induced by IL-2 and diminished the early apoptosis of cells. Moreover, culture supernatants derived from PBMCs treated with Trappin-2 (48 h) showed a slight increase in IL-4 production, what was confirmed by intracellular staining of IL-4 on CD3 T cells. Besides, Trappin-2 significantly increased GATA-3 expression on PBMCs as determined by Western Blot. However, the most significant result was a clear increase in IL-9 expression on CD4 T cells, after 48 h of culture of cells with Trappin-2. Moreover, the expression of PU.1 was also increased on Trappin-treated cells. Finally, TGF-β was measured in the culture supernatants of PBMC treated with Trappin-2, given that naive CD4+ T cells differentiate in vitro into IL-9-secreting cells by effect of IL-4 and TGF-β. The results obtained showed that Trappin-2 in fact increased TGF-β at 48 h of culture. Overall, our findings suggest that Trappin-2 induces a cytokine microenvironment compatible with a potential Th9 pattern.