Gene Expression Profiling in Kidney Transplant Correlation with DGF

GUERRIERI DIEGO; RE LUIS; PETRONI JORGELINA; CHULUYAN EDUARDO; INCARDONA CLAUDIO; CASADEI DOMINGO

FILADELFIA

Congreso; American Transplantation Congress 2011; 2011

Ischemia reperfusion injury (IRI) is a coordinated process leading to delayed graft function (DGF) and reduced long-term survival of the transplanted organ. Despite recent advances in immunosuppressive therapy, delayed graft function (DGF) remains an important problem after kidney transplantation. Different studies have related various clinical factors to DGF, such as donor age, recipient age, cold ischemia time, initial immunosuppressive regimens. The aim of present study was to examine the changes in gene expression profile in graft renal after 7 days of KTx in relation to the development of delayed graft function. MATERIAL AND METHODS: The study included 16 recipients of cadaveric renal grafts (11 males, 5 females)-8 with DGF and 8 without DGF. Levels of gene expression after 7 days of transplantation were quantified by Real-Time PCR array. RESULTS: The increased expression of Tgf-b and CD14 were observed in patients without DGF. Meanwhile, IL-6, IL-1, CCR3, CD1d, IRAK2 were downregulated in patients without DGF. However, there were no statistically significant correlation between those markers and the DGF duration. CONCLUSIONS: A systems biological assessment of these molecular changes suggests that processes along inflammation are of pivotal importance for the early stage of IRI. In deceased-donor kidney transplantation as the primary causative factor resulting in IRI and DGF, a distinct signature and choreography of molecular events in the graft before harvesting seems to be associated with subsequent DGF