INVESTIGADORES
GUERRIERI Diego
congresos y reuniones científicas
Título:
THE ACTION OF SLPI ON TUBULAR EPITHELIAL CELLS AND IMMUNE RESPONSE PROTECS THE KID- NEY OF TRANSPLANTNT PATIENTES.
Autor/es:
AMBROSI NELLA; REMOLINS CARLA; ROMEO HORACIO; UVA PABLO; RE LUIS; INCARDONA CLAUDIO; CHULUYAN EDUARDO; GUERRIERI DIEGO
Lugar:
BUENOS AIRES
Reunión:
Congreso; REUNIÓN CONJUNTA SAIC SAI AAFE NANOMED.AR; 2021
Institución organizadora:
SAIC SAI
Resumen:
Secretory leukocyte protease inhibitor (SLPI) is a serine proteaseinhibitor with pleitropic activities on immune and non-immune cells.Lately, it has been proposed SLPI as a biomarker of acute kidneyinjury with a protective role on tubular epithelial cells. The aim of thepresent work was to unravel whether SLPI action on kidney is animmune mediated effect. We found that the administration of SLPIreduced the kidney damage in a rat model of ischemia reperfusioninjury. However, SLPI was also able to reduce the damage in a gen-tamicin-induced nephrotoxicity rat model. In in vitro experiments, theHK-2 human epithelial cells-treatment with SLPI reduced the cellapoptosis induced by serum starving conditions or calcineurin in-hibitors. A real-time PCR array data with HK-2 cells revealed thatSLPI modulated transcript levels of genes mainly involved in DNArepair, response to misfolded proteins and inflammatory responsepathways. Furthermore, SLPI favors the proliferation and migrationof HK-2 cells. In kidney transplant patients, plasma levels of SLPIwere high and indirectly associated with kidney function but alsowith leukocyte proliferation index. However, the levels of SLPI tran-scripts found in the renal biopsies were indirectly associated withplasma creatinine and TGFb but directly associated with HMOX-1.An indirect association was found between plasma levels of FK506and SLPI. These results confirm that local SLPI exert a protectiverole in kidney transplant patients which can be mediated directlyon epithelial cells and indirectly by affecting the immune response.Furthermore, it suggests that immunosuppression treatment can re-duce the expression of this nephropotective factor.