PROTECTIVE EFFECT OF IMMUNOSUPPRESSIVE TREATMENT BEFORE ORTHOTOPIC KIDNEY

CICORA FEDERICO; LAUSADA NATALIA; VAZQUEZ DANIELA; CICORA PAOLA; GUERRIERI DIEGO; GONZALEZ PEDRO; SALAZAR GUSTAVO; STRINGA PABLO; RAIMONDI CLEMENTE

TRANSPLANT IMMUNOLOGY

ELSEVIER SCIENCE BV

Año: 2011 vol. 24 p. 107 - 112

0966-3274

Background: Ischemia reperfusion injury (IRI) is one of the risk factors for delayed graft function, acute rejection and long term allograft survival after kidney transplantation. It’s an independent antigen inflammatory process that produces tissue damage. Our objective was to study the impact of immunosuppressive treatment (IS) on IRI applying only one dose of IS before orthotopic kidney autotransplantation, Methods: Twenty-four rats allocated in four groups were studied. One group served as control (G1: autotransplanted rats without IS) and the rest received IS 12 h before kidney autotransplantation (G2: Rapamycin, G3: Mycophenolate mofetil and G4: Tacrolimus). Results: Improved renal function and systemic inflammatory response were found among IS groups compared with control group (Delta Urea p<0.0001; Delta Creatinine p<0.0001; Delta C3 p<0.001). The number of apoptotic nuclei in renal medulla in G1 was higher than in IS groups (p<0.0001). Tubular damage was less severe in IS groups respecting G1 (p<0.001). C3, TNF-á and IL-6 expression in kidney samples were reduced when IS was used compared with control group. No differences were observed among the different immunosuppressive drugs tested. Conclusions: These data suggest that the use of IS administered before transplant attenuates IRI process after kidney transplantation in an animal model.