Sexual dimorphism in the immunometabolic profile of Hofabuer cells

DANIEL PAPARINI; ESTEBAN GRASSO; FRANCO AGUILERA; BRENDA LARA; M. AGUSTINA ARSLANIAN; VICTORIA LELLA; VANESA HAUK; FATIMA MERECH; CLAUDIO SCHUSTER; SOLEDAD GORI; CESAR MELLER; MARCELO MARTÍ; ROSANNA RAMHORST; DAIANA VOTA; CLAUDIA PÉREZ LEIRÓS

Congreso; LXXI REUNIÓN CIENTÍFICA ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI); 2023

SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI)

Aims: Hofbauer cells (HB) are the only foetal immune cell population within thestroma of healthy placenta. However, the functional properties of these cells arepoorly described. HB are transcriptional and chromosomically similar to yolk sacmacrophages. Here we studied the effect of sexual dimorphism in theimmunometabolism of human placental HBat term.Methodology: HB cells were obtained from placental tissue by enzymaticdigestion from healthy pregnant women at term along with autologous PBMCs.HB were cultured overnight and the phenotypic profile, efferocytosis, glucoseuptake and lipid droplets accumulation were evaluated by flow cytometry andELISA. We have started with bioinformatic analysis in public database tounderstand the differential imprinting of placentas with male or female newborns.Results: HB from female placenta (fHB) present higher production ofantiinflammatory markers CD163, CD206 and CD39 accompanied by lessersecretion of IL-1 than HB from male placentas (mHB) (*P