Homeostasis loss in VIP deficient mice is associated with altered regulatory T cells recruitment

LUCILA GALLINO; VANESA HAUK; ESTEBAN GRASSO; MARCOS PALLIGAS; NORA SARACO; ESPERANZA BERENSZTEIN; NATALIA PASCUALI; FERNANDA PARBORELL; JAMES WASCHEK; CLAUDIA PÉREZ LEIRÓS; ROSANNA RAMHORST

Buenos Aires

Congreso; Internation Federation of Placenta Association meeting 2019, VIII SLIMP; 2019

Internation Federation of Placenta Association, Soc Latinoamericana de interaccion materno-fetal y placenta

Objectives: In order to have a successful pregnancy, it is required a balance between proinflammatory and tolerogenic mediators. Since VIP (vasoactive intestinal peptide) is a master regulator associated with Tregs induction and recruitment, we aim to understand the relevance of VIP in normal pregnancy with focus on the Tregs.Methods: We used two mice models: VIP Knockout and Foxp3-IRES-GFP, both in a C57 background. The serum and tissues obtained were analyzed by flow cytometry, RT-PCR and histological staining. We also performed Foxp3-adoptive cell transfer and migration assays.Results: On the day of estrus we found histological differences between the ovaries and uterus of a WT mice vs VIP knockout mice (-/-). In uterus, these changes were accompanied with undetectable levels of expression Foxp3 in the (-/-) group, higher expression of RORgt and a decrease in IL-10 (p