The decidualization by VIP conditions endometrial receptivity and immune microenvironment through Reticular Stress and Unfolded Protein Response

SOLEDAD GORI; ELIZABETH SOCZEWSKI; ESTEBAN GRASSO; LAURA FERNÁNDEZ; GALLINO, L.; MARCELA IRIGOYEN; THALITA LOBO.; GABRIELA SALAMONE; ROSIANE MATTAR; SILVIA DAHER; ROSANNA RAMHORST

Buenos Aires

Congreso; Internation Federation of Placenta Association meeting 2019, VIII SLIMP; 2019

Internation Federation of Placenta Association, Soc Latinoamericana de interaccion materno-fetal y placenta

Objectives: The vasoactive intestinal peptide (VIP) induces the decidualization program and conditions the immunoregulation of the implantation process. Endometrial stromal cells undergo reticular stress (RS) and unfolded protein response (UPR), which will allow them to induce a physiological sterile inflammatory response with IL-1b production. Here we focus on VIP effect as decidualization inducer through the RS and UPR pathways and whether modulates inflammation and angiogenesis. Methods: We used human endometrial stromal cell line (HESC) differentiated with VIP (10-7M) or with medroxyprogesterone (MPA 10-6M) and dibutyryl-cAMP (2,5 10-3M). To functionally studies we used and in vitro implantation model based on the generation of Blastocyst-like spheroids from Swan-71 cells, transferred on monolayer HESC-decidualized cells.Results: VIP significantly increased the expression of ATF6, a RS-sensor (p