Decidualization process conditions myeloid regulatory cells

SOLEDAD GORI; ELIZABETH SOCZEWSKI; ESTEBAN GRASSO; LAURA FERNÁNDEZ; ANA SCHAFIR; JORGELINA BLEJER; ALEJANDRA GRASSI BASSINO; CLAUDIA PÉREZ LEIRÓS; GABRIELA SALAMONE; ROSANNA RAMHORST

Tucuman

Congreso; LXVII Reunión anual de la Sociedad Argentina de Inmunología; 2019

Sociedad Argentina de Inmunología

DC-10 is a novel and distinctive subset of tolerogenic dendritic cell (DC) characterized to be a potent IL-10 producer and type 1 Tregs (Tr1)-inducer. Although DC-10 and others subsets of Myeloid regulatory cells (Mregs) were recently found increased at the human decidua, their role in materno-fetal tolerance maintenance are still unknown. Due to decidualization process involves immunoregulatory factors secretion by endometrial stromal cells, we focus on the impact of this process in conditioning monocytes to Mregs subsets. Thus, conditioned media (CM) of Human endometrial stromal cell line (HESC) decidualized with MPA+dbcAMP for 8 days (Dec-CM) or not (Non-dec CM) were collected. Then, isolated monocytes from peripheral blood mononuclear cells of healthy donors were cultured with rhGM-CSF+rhIL-4 for 5 days in the absence/presence of CM. We observed that Dec-CM was able to inhibit CD14+ cellsdifferentiation to immature DC (p