PROGESTERONE AND VIP CROSS-TALK ON TROPHOBLAST CELL FUNCTION: FOCUS ON THEIR PHAGOCYTIC ABILITY

MARIANA AGÜERO; DAIANA VOTA; ESTEBAN GRASSO; LUCILA GALLINO; ELIZABETH SOCZEWSKI; CLAUDIA PÉREZ LEIRÓS; ROSANNA RAMHORST

Mar del Plata

Simposio; VI Latin American Symposium on Maternal-Fetal Interaction and Placenta (VI SLIMP) and the V Latin American Symposium on Reproductive Immunology (V LASRI); 2015

Vasoactive intestinal peptide (VIP) is produced by trophoblast cells and displays multiple target circuits that allow immune tolerance to pregnancy-related antigens. However, its interaction with progesterone (P4) in this system is still unknown. Objective: We aimed to evaluate the immunomodulatory effects of P4 and VIP on trophoblast cell function focusing on their phagocytic ability. Methods: We used an in vitro model based on the Swan71 cell line cocultured or not with PBMC from fertile women and evaluated nitrites (by Griess Method), MMP9 (by Zymography) and ROS production by FACS, COX-2 and VPACs expression (by RT-PCR). Phagocytic ability was quantified using latex-beads FITC-conjugated (efferocytosis) or apoptotic red blood cells CFSE-stained (eryptosis) by FACS and cytokine production by RT-PCR. Results: After the interaction between Swan cells and maternal PBMC in the presence of VIP (10-7M) and P4 (10-4M), nitrites production, MMP9 activity and COX-2 expression decreased (p