Modulation of maternal LIF producers T cells by trophoblast and paternal antigens.

LAURA FRACCAROLI; ESTEBAN GRASSO; ELENA ZEITLER; EDUARDO LOMBARDI; SEBASTIAN GOGORZA; JUAN JOSE ETCHEPAREBORDA; CARLOS NAGLE; MARTA CORTELEZZI; CLAUDIA PÉREZ LEIRÓS; ROSANNA RAMHORST

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Copenhagen; Año: 2011 vol. 65 p. 133 - 145

1046-7408

The allorecognition in uterus enhances the production of essential growth factors for embryonic development, such as leukemia inhibitory factor (LIF), neuropeptides, as vasoactive intestinal peptide (VIP), and progesterone modulate the pro-inflammatory response during the feto-maternal dialogue. Here, we evaluated the contribution of maternal CD4 cells, activated by paternal or trophoblast antigens, to LIF production and its modulation by VIP and progesterone in fertile women or with recurrent spontaneous abortions (RSA). The frequency of fertile CD4+ LIF+ cells was increased by VIP and progesterone after the co-culture with paternal alloantigens or with an immortalized trophoblast cell line (Swan 71). This increase was accompanied by a decrease in MMP-9 gelatinolytic activity and an increase in the pSTAT3/STAT3 ratio. Although under the same conditions, patients with RSA have decreased levels of LIF expression and these could not be modulated by VIP and progesterone. Moreover, these patients have a reduced number of endometrial infiltrated CD4+ LIF+ cells in comparison with fertile women which could be related with the exacerbated inflammatory response observed in the maternal–fetal dialogue model.