Initial Insights into the Genetics Characterization of Actinomycetales Isolated from Soil in the Province of Misiones, Argentina.

CENTENO CELIA; MARTINA PABLO; VALLE LISBOA SILVINA; KLEINBIELEN TAMARA; URQUIZA J; FERRERAS JA

Foz de Iguazu

Congreso; XXII International Congress of Genetics; 2018

Areas of the world with high concentration of biodiversity are an important source for the discovery of new natural products with important biological activities. Misiones, in Argentina, contains part of the Atlantic forest and it is one of the most biodiverse region of the country. However, microbial biodiversity still remains to be explored in this area. Among bacteria, Actinomycetales are known to be an important source of natural products and harbor many cryptic pathways for these metabolites. As we expect that microbial biodiversity correlate with the observed biodiversity of higher organism, we anticipate we can isolate new strains with the potential capacity to produce novel natural products.The main goal of this work is to generate and characterize genetically, morphologically and in its capacity to produce natural products, a collection of strain of the order Actinomycetales isolated from different soils samples collected in the province of Misiones, Argentina. Different soils samples were suspended in sterile water and dilutions were plated in an agar medium supplemented with ?soil extracts? and the antibiotics hygromycin and nalidix acid. Single colonies were picked between 14 and 30 days of culture at 28C. Genetic identification was performed by rRNA 16S sequencing and and rpoD as secondary marker for some of them. Here we report the initially genetic characterization of a group of 20 isolates with their phylogenetic relationships, showing the great potential of this area for the discovery of new bacteria with the capacity to produce novel natural compounds.Funding Agency: Agencia de Promoción Científica y Técnica. Argentina