Burkholderia cepacia complex: 11 years of surveillance in patients with Cystic Fibrosis in Posadas, Argentina

MARTINA PABLO; MARTINEZ M; RIVAS SEBASTIÁN; LEQUIZAMÓN LORENA; VON SPECHT, MARTHA; FERRERAS JA

REVISTA ARGENTINA DE MICROBIOLOGíA

ASOCIACION ARGENTINA MICROBIOLOGIA

Lugar: Buenos Aires; Año: 2019

0325-7541

Cystic fibrosis patients with Burkholderia cepacia complex pulmonary infections havehigh morbidity and mortality. Worldwide, this disease is undergoing substantial epidemiologicalchanges. Advances in the diagnosis and treatment have conditioned an increase in child survivalas well as in the proportion of affected adults. In order to know our reality, we refer toan epidemiological study in 64 CF patients during 11 years of surveillance, focusing on infectionscaused by Burkholderia species. Conventional and automated phenotypic tests, restrictionfragment length polymorphism-recA, recA gene sequencing, and matrix-assisted laser desorptionionization-time of flight (MALDI-TOF) mass spectrometry were applied. Bacterial isolateswere also tested for antimicrobial susceptibility patterns. The prevalence of Burkholderia cepaciacomplex was 9.4%. Based on recA gene sequencing, the most common species identifiedwere Burkholderia cenocepacia (67.3%) and Burkholderia vietnamiensis (20.3%). Ceftazidimeand meropenem were the most active, inhibiting 53% and 46% of isolates, respectively. Thisreport represents the first systematic study of Burkholderia infections in our CF population sincebeginning of monitoring and treatment and highlights the importance of continued longitudinalstudies.