INVESTIGADORES
STROBL MAZZULLA Pablo Hernan
artículos
Título:
Histone demethylase JmjD2A regulates neural crest specification
Autor/es:
STROBL MAZZULLA, P.H.; SAUKA-SPENGLER, T.; BRONNER-FRASER, M.
Revista:
DEVELOPMENTAL CELL
Editorial:
CELL PRESS
Referencias:
Año: 2010 vol. 19 p. 460 - 468
ISSN:
1534-5807
Resumen:
The neural crest is a multipotent stemcell-like populationthat is induced during gastrulation, but onlyacquires its characteristic morphology, migratoryability, and gene expression profile after neurulation.This raises the intriguing possibility that precursorsare actively maintained by epigenetic influences ina stem cell-like state. Accordingly, we report thatdynamic histone modifications are critical for propertemporal control of neural crest gene expressionin vivo. The histone demethylase, JumonjiD2A(JmjD2A/KDM4A), is expressed in the forming neuralfolds. Loss of JmjD2A function causes dramaticdownregulation of neural crest specifier genesanalyzed by multiplex NanoString and in situ hybridization.Importantly, in vivo chromatin immunoprecipitationreveals direct stage-specific interactions ofJmjD2A with regulatory regions of neural crest genes,and associated temporal modifications in methylationstates of lysine residues directly affected by JmjD2Aactivity. Our findings show that chromatin modificationsdirectly control neural crest genes in vertebrateembryos via modulating histone methylation.
