INVESTIGADORES
STROBL MAZZULLA Pablo Hernan
artículos
Título:
DNA methyltransferase3A as a molecular switch mediating the neural tube-to-neural crest fate transition
Autor/es:
HU, N.; STROBL MAZZULLA, P.H.; SAUKA-SPENGLER, T.; BRONNER, M.
Revista:
GENES & DEVELOPMENT.
Editorial:
COLD SPRING HARBOR LAB PRESS
Referencias:
Año: 2012 vol. 26 p. 2380 - 2385
ISSN:
0890-9369
Resumen:
Here, we explore whether silencing via promoter DNA methylation plays a role inneural versus neural crest cell lineage decisions. We show that DNA methyltransferase3A(DNMT3A) promotes neural crest specification by directly mediating repression of neuralgenes, like Sox2 and Sox3. DNMT3A is expressed in the neural plate border and itsknockdown causes ectopic Sox2 and Sox3 expression at the expense of neural crestmarkers. In vivo ChIP of neural folds demonstrates that DNMT3A specifically associateswith CpG islands in the Sox2 and Sox3 promoter regions, resulting in their repression bymethylation. Thus, DNMT3A functions as a molecular switch, repressing neural to favorneural crest cell fate.