IAPT chromosome data 31

CHIARINI, F. E.; KNAPP, SANDRA; OREJUELA, ANDRÉS

TAXON

INT ASSOC PLANT TAXONOMY

Año: 2019 vol. 68 p. 1374 - 1380

0040-0262

We present here chromosome numbers and karyotype features of seven species from four genera of Solanaceae. Mitotic chromosomes were examined in root tips obtained from germinating seeds. Roots were pretreated in saturated p-dichlorobenzene in water for 2 h at room temperature, fixed in 3 : 1 ethanol : acetic acid, washed in distilled water, digested 45 min at 37°C with Pectinex SP ULTRA (Novozymes, Bagsværd, Denmark), and squashed in a drop of 45% acetic acid. Slides were stained with Giemsa. Six of these chromosome counts are first reports for those taxa, while the remaining count confirms previously published data. All species were presumed diploid, and they have chromosomes of medium size (c = from 2.34 to 5.45 μm), falling within the range expected for the family Our data are useful for distinguishing species and to characterize some clades within a large family such as the Solanaceae, where chromosome morphology has already proven to be a useful approach. More counts and karyotypes, especially in early-branching Solanaceae clades, are needed in order to establish firm hypotheses of chromosome evolution across this economically important family.