INVESTIGADORES
CHIARINI Franco Ezequiel
artículos
Título:
Genome size and karyotype diversity in Solanum sect. Acanthophora (Solanaceae)
Autor/es:
CHIARINI, F.; SANTIÑAQUE, F.; URDAMPILLETA, J.; LAS PEÑAS, M.L.
Revista:
PLANT SYSTEMATICS AND EVOLUTION
Editorial:
SPRINGER WIEN
Referencias:
Lugar: Viena; Año: 2013 p. 1 - 12
ISSN:
0378-2697
Resumen:
In order to study the evolution of different cytogenetic characters in species of Solanum sect. Acanthophora in relationship to the known phylogeny for this group, the following techniques were used: CMA/DAPI chromosome banding; fluorescent in situ hybridization with probes for the 18-5.8-26S and the 5S rDNA genes in mitotic chromosomes; nuclear DNA quantification by flow cytometry. Depending on the species, 2?6 of the 12 basic chromosome pairs were identified. The heterochromatic banding patterns were shown to be species-specific. All species presented one chromosome pair bearing a 18-5.8-26S signal and one pair (rarely two) with a 5S signal, the two rDNA sites being non-syntenic. The techniques employed allowed us to stablish two species groups within sect. Acanthophora: one with small, symmetric chromosomes, little heterochromatin and lower DNA content, and the other one with larger and more asymmetric chromosomes, more heterochromatin CMA+/DAPI- (associated with NOR or not) and a higher DNA content. An elevated karyotype asymmetry would be associated with a high amount of heterochromatin and a high DNA content. The trend within sect. Acanthophora would be towards a loss of heterochromatin, a reduction of chromosome size, and an increase in symmetry.