INVESTIGADORES
KLINKE Sebastian
congresos y reuniones científicas
Título:
Search and discovery of new low-temperature active enzymes from Antarctic microorganisms (comunicación oral)
Autor/es:
MARTIN ARAN; LEONARDO PELLIZA; SEBASTIÁN KLINKE; JIMENA RINALDI; GASTÓN PARIS; CLARA SMAL; MARIANA GALLO; ANDRÉS BERCOVICH; WALTER MAC CORMACK; FERNANDO A. GOLDBAUM; ADRIÁN TURJANSKI; DANIEL O. CICERO
Lugar:
Frauenchiemsee
Reunión:
Congreso; 35th FGMR Discussion Meeting & Joint Conference of the German, Italian and Slovenian Magnetic Resonance Societies; 2013
Institución organizadora:
German Magnetic Resonance Society
Resumen:
Cold-adapted organisms and their enzymes offer a large variety of real and potential applications in different fields of biotechnology. With the aim of discovering new enzymes with activity at low temperatures we undertook the analysis of proteins belonging to Bizionia argentinensis (BA), a newly discovered bacterium [1]. A particularly convenient protocol based on NMR experiments [2] was set up in order to identify the best candidates for structural determination. In this way, more than 30 proteins were classified as good candidates for three-dimensional structure determination. The hypothesis is that although the amino acidic sequence is not conserved, structural homology will allow the functional classification of the proteins and the discovery of new enzymes. We have already determined the structure of two proteins with unknown function belonging to BA: BA42 (by NMR) and C24 (by X-ray crystallography). In this talk, the first insights into the possible biological function of the these two newly discovered proteins will be presented. [1] Lanzarotti E. et al., J. Bacteriol., 193 (2011), 6797-8. [2] Galvão-Botton, L.M.P., Katsuyama, A.M., Guzzo, C.R., Almedia, F.C.L., Farah, C.S. and Valente, A.P. FEBS Letters, 552 (2003), 207-213