Sequencing Solanum pennellii – the wild parent of tomato introgression lines which harbour an unprecedented access to systems biology in crop plant species

BOCK R.; CARRARI F.; KAMENETZKY L.; FERNIE A. R.; USADEL B.; STITT M.; WILLMITZER L.

Proyecto Internacional

Otro; Secuenciación del genoma del tomate silvestre (Solanum pennellii); 2008

The resolution offunctional genomic analyses is only as powerful as the diversity ofphenotypes that are associated with genomesequences. The focus of this proposal is the sequencing of Solanum pennellii (~1200 Mb), which is the wild speciesdonor parent of the extensively phenotyped tomato introgression line (IL) population. The impetus for this projectis to leverage the rich complex trait variation exposed in the S. pennellii ILs over the last 18 years, as well asthe widespread research in international Solanaceae projects. The ILs comprise the most diverse inter-specificquantitative trait mapping population in any system, and are composed of 76 immortalized nearly isogenic lines, eachcarrying a single marker-defined exotic chromosome segment, developed through a succession of backcrosseswith a variety of tomato, S. lycopersicum. Solanaceae researchers worldwidehave utilized the publicly available fullgenome coverage ILs to map more than 2500quantitative trait loci (QTL) affecting plant biomass, yield, droughttolerance,morphology, metabolism and gene expression. We request additionally funding to get the genome ofS. pennellii sequenced commercially and also to assemble and mine the richdata source provided at bothbioinformatic and functional levels.