INVESTIGADORES
KAMENETZKY Laura
congresos y reuniones científicas
Título:
Physical map of quantitative metabolic loci from wild species tomato (Solanum pennellii)
Autor/es:
KAMENETZKY L.; ASIS R.; GONZÁLEZ V.; VAN SLUYS M.A.; GIOVANNONI J.; FERNIE A. R.; ROSSI M.; CARRARI F.
Lugar:
México
Reunión:
Congreso; Plant Biology; 2008
Resumen:
The adoption of tomato varieties selected for their high performance in terms of harvest index has considerably reduced accessibility to genetic basis of the Solanum genus. This fact has negatively impacted on the production of alimentary and pharmacological compounds. The main objective of this work is to determine the genetic bases of key points regulating tomato fruit metabolism. We present here the construction of a physical map along 5 genomic regions containing metabolic QTL previously mapped on an introgression line population (S. lycopersicum x S. pennellii). Two genomic libraries from S. pennellii were double-screened with all QTLs co-located markers available at the Tomato Genome Consortium (www.sgn.cornell.edu). End-sequence analyzes of all anchored clones including gene annotation, contigs assembling and comparison with the orthologous region of S. lycopersicum allowed the construction of physical maps. Full length sequences of selected clones containing candidates genes associated with the observed metabolic traits are being generated. The results obtained here will be presented in the context of the Tomato Genome Project and would render the identification of wild alleles potentially involved in the tomato fruit quality determination.