INVESTIGADORES
KAMENETZKY Laura
congresos y reuniones científicas
Título:
High inter- and intra-strain polymorphism in genes encoding Antigen B from Echinococcus granulosus
Autor/es:
KAMENETZKY L.; MUZULIN P. M.; GUTIERREZ A. M.; ANGEL S. O.; ZAHA A.; GUARNERA E. A.; ROSENZVIT M. C.
Lugar:
Misiones, Argentina
Reunión:
Congreso; XI Reunion de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB); 2004
Institución organizadora:
Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB)
Resumen:
Echinococcus granulosus, the causative agent of cystic hydatid disease, exists as a number of intraspecific variants that differ in biological characters, such as intermediate host specificity and infectivity to humans. One of the major antigens of the larval stage of the parasite, the heterodimeric antigen B (AgB), is widely used in diagnosis and has been involved in the evasion of host immune response. We used PCR-SSCP followed by sequencing to evaluate the intraspecific variation and expression of genes of the AgB family. Forty two genomic sequences were isolated and clustered in five genes (B1 to 5) by maximum parsimony analysis. The variants were differentially distributed among strains. Neutrality was rejected for AgB2 sequences. Intra-strain variation was also observed, mainly in sheep strain. The level of nucleotide and amino acid variation observed was higher than the reported so far for coding genes of other helminth parasites. Differential expression of AgB variants between protoscoleces of sheep and pig strains was detected by RT-PCR. These data demonstrate for the first time the genetic variability of antigen-coding genes among genetically characterised strains of E. granulosus.