INVESTIGADORES
KAMENETZKY Laura
artículos
Título:
The cytosolic invertase NI6 affects vegetative growth, flowering, fruit set and yield in tomato
Autor/es:
COLUCCIO LESKOW, C.; CONTE M.; POZO, T.; BERMÚDEZ L.; LIRA, B.; GRAMEGNA,G.; BAROLI, I.; BURGOS, E.; ZAVALLO, D.; KAMENETZKY L; ASIS R.; GONZALEZ, M.; ALISDAIR, F.; ROSSI M.; CARRARI, F.
Revista:
JOURNAL OF EXPERIMENTAL BOTANY
Editorial:
OXFORD UNIV PRESS
Referencias:
Lugar: Oxford; Año: 2020
ISSN:
0022-0957
Resumen:
Sucrose metabolism is of high importance for most plant species, both as the main source of carbon and via signaling mechanisms that have been proposed for this molecule. Two cleaving enzymes channel sucrose into sink metabolism; sucrose synthases (SUS) and invertases (INV), which are localized in different subcellular compartments. Although acid soluble and insoluble invertases have been largely investigated, studies on the role of neutral invertases (A/N-INV) have lagged behind. Here, we identified a tomato A/N-INV encoding gene (NI6) co-localizing with a previously reported pathway QTL largely affecting primary carbon metabolism in tomato. Of the eight A/N-INV genes identified in the tomato genome, NI6 mRNA is present in all organs, but its expression washigher in sink tissues (mainly roots and fruits). A NI6-GFP fusion protein was found in the cytosol of mesophyll cells. Tomato NI6-silenced plants showed impaired growth phenotypes, delayed flowering and dramatic reduction in the fruit set. Global gene expression and metabolite profile analyses of these plants revealed that NI6is not only essential for sugar metabolism but also plays a signaling role in stress adaptation. Gene-metabolite network analyses allowed identification of major hubs, whose expression patterns were greatly affected by NI6silencing, within the signaling cascade that coordinates carbohydrate metabolism with growth and development in tomato