Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces

CARNEVALE, S.; PANTANO, M.L.; KAMENETZKY, L.; MALANDRINI, J.B; SORIA, C. C; VELÁSQUEZ, J.N.

ACTA PARASITOLOGICA

VERSITA

Lugar: Varsovia; Año: 2015

1230-2821

Fasciolosis is an important parasitic zoonosis considered the most important helminth infection of ruminants in tropicalcountries. The aim of this study was to develop a PCR assay for the sensitive and specific detection of F. hepatica in formalinpreserved sheep faeces. A 405-bp fragment of the cytochrome c oxidase subunit 1 gene of F. hepatica was amplified from stoolsamples of infected sheep. The PCR assay showed a detection limit of 20 pg of F. hepatica DNA. No cross-reactions wereobserved with samples containing coccidian oocysts or gastrointestinal nematodes eggs. Our PCR technique showed to beeffective for specific detection of F. hepatica infections in sheep.