Echinococcus granulosus: DNA Extraction from Germinal Layers Allows Strain Determination in Fertile and Nonfertile Hydatid Cysts

KAMENETZKY L.; CANOVA S. G.; GUARNERA E. A.; ROSENZVIT M. C.

EXPERIMENTAL PARASITOLOGY

Elsevier

Año: 2000 vol. 95 p. 122 - 127

0014-4894

A method for the isolation of Echinococcus granulosusDNAfrom germinal layers of hydatid cysts is described. The method includes a hexadecyltrimethylammonium bromide/chloroform extraction and an adsorption to diatomaceous earth suspension. DNA suitable for polymerase chain reaction was obtained and used for parasite strain determination by mitochondrial cytochrome c oxidase I gene sequencing. Fertile and nonfertile cyst isolates from sheep, cattle, pigs, and humans were characterized. Hitherto, no direct parasite strain characterization has been made on nonfertile hydatid ysts, whereas here we report that nonfertile hydatid cysts were pro- duced by sheep strain (G1 genotype) in sheep, cattle, and humans and by pig strain (G7 genotype) in pigs.