INVESTIGADORES
RODRIGUEZ Gustavo Ruben
congresos y reuniones científicas
Título:
Identification of QTNs controlling a domestication trait in tomato
Autor/es:
VAN DER KNAAP, ESTHER; ZHANG, NA; RODRÍGUEZ, GUSTAVO RUBÉN; HUANG, ZEJUN
Lugar:
Neuchâtel
Reunión:
Conferencia; SOL 2012, 9th Solanaceae Conference: From the Bench to Innovative Applications; 2012
Institución organizadora:
Solgenomics network
Resumen:
One of the most important domestication traits of tomato (Solanum lycopersicum) is increasedfruit mass. Mapping populations derived from crosses between cultivated tomato and its closest wild relative, S. pimpinellifolium, revealed a number of QTL that control fruit mass. We fine-mapped fw3.2 and fw11.3, and partially sequenced the loci in a core collection of tomato accessions developed for association mapping of genes that underlie domestication. Using TASSEL, we identified one SNP in the promoter of the candidate gene at fw3.2 that is significantly associated with fruit mass. Further genetic analysis of populations that segregated for the SNP at the fw3.2 locus demonstrated that fruit mass is indeed associated with the allele of the critical SNP in two out of five populations. For fw11.3, the region was narrowed down to a 14 kb interval comprised of four candidate genes and many polymorphisms that distinguish the parents. Fortuitously, fw11.3 also segregated in an intraspecific population with fewer polymorphisms between the parents. Association mapping of those polymorphisms showed that the most significant QTN is a deletion in cultivated tomato of part of one of the candidate genes resulting in a dominant negative phenotype. These findings support the notion that association mapping in tomato leads to the identification of the QTN underlying the locus. This work is supported by NSF DEB 0922661