Comparación de los perfiles metabólicos obtenidos por 1H-NMR y GC-MS en frutos de tomate producidos por una población derivada de un programa de mejoramiento genético

ZANOR, MARIA INES; LOPEZ, MARIANA; BOGGIO, SILVANA B; SORREQUIETA, AUGUSTO; PRATTA, GUILLERMO RAUL; RODRÍGUEZ, GUSTAVO RUBÉN; ZORZOLI, ROXANA; PICARDI, LILIANA AMELIA; CARRARI, FERNANDO; VALLE, ESTELA M

Puerto Madryn

Congreso; XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2010

Tomato (Solanum lycopersicum L.) is a world-wide horticultural crop and a model plant for research on fruit metabolism. Data are emerging about the use of robust analytical platforms such as nuclear magnetic resonance spectroscopy (NMR) and gas chromatography-mass spectrometry (GC-MS) for global metabolite analysis of biological systems. The aim of this work was to examine the metabolome of tomato fruits with different organoleptic qualities by coupling NMR and GC-MS data through multivariate analysis. These fruits were obtained by crossing the domesticated S. lycopersicum (cv Caimanta) with a wild relative S. pimpinellifolium species. This later one is appreciated for its sweetness and stress-tolerance. Correlative analyses between metabolite signals were performed by principal component analysis. Results suggested that a substantial number of significantly correlating metabolites were qualitative- and quantitatively similar in the fruits, suggesting that both platforms complement each other. Examples are primary metabolites involved in the taste such as hexoses (fructose and glucose), organic acids (citrate and malate), and amino acids (alanine, aspartate, glutamate, valine and threonine). Our strategy provides complementary information about the potential of NMR and GCMS as global metabolite fingerprinting analytical technologies for the study of tomato fruit quality.Solanum lycopersicum L.) is a world-wide horticultural crop and a model plant for research on fruit metabolism. Data are emerging about the use of robust analytical platforms such as nuclear magnetic resonance spectroscopy (NMR) and gas chromatography-mass spectrometry (GC-MS) for global metabolite analysis of biological systems. The aim of this work was to examine the metabolome of tomato fruits with different organoleptic qualities by coupling NMR and GC-MS data through multivariate analysis. These fruits were obtained by crossing the domesticated S. lycopersicum (cv Caimanta) with a wild relative S. pimpinellifolium species. This later one is appreciated for its sweetness and stress-tolerance. Correlative analyses between metabolite signals were performed by principal component analysis. Results suggested that a substantial number of significantly correlating metabolites were qualitative- and quantitatively similar in the fruits, suggesting that both platforms complement each other. Examples are primary metabolites involved in the taste such as hexoses (fructose and glucose), organic acids (citrate and malate), and amino acids (alanine, aspartate, glutamate, valine and threonine). Our strategy provides complementary information about the potential of NMR and GCMS as global metabolite fingerprinting analytical technologies for the study of tomato fruit quality.