Protein profiling in F1 and F2 generations of two tomato genotypes differing in ripening time

RODRÍGUEZ, GUSTAVO RUBÉN; SEQUIN LUCIANA,; PRATTA, GUILLERMO RAUL; ZORZOLI, ROXANA; PICARDI, LILIANA AMELIA

BIOLOGIA PLANTARUM

SPRINGER

Año: 2008 vol. 52 p. 548 - 552

0006-3134

Pericarp polypeptide profiles were analyzed at three ripening stages (MG, BR and RR) in the F1 hybrid and the F2 population from the cross between the accessions: LA1385 (L. esculentum var. cerasiforme) and 804627 (L. esculentum, a homozygous genotype for the nor mutant). The objective of this analysis was to characterize and identify differential expression of proteins involved in tomato ripening. Six polymorphic polypeptides were observed in LA1385, while no polymorphic polypeptides among ripening stages was observed in 804627. On the other hand, some polypeptides in the F1 hybrid were not observed in the parents whereas others were present in both parental genotypes and were unnoticeable in the hybrid genotype. From a cluster analysis on the protein profiles of the F2 population, the differential expression of proteins allowed to distinguish MG from the others two stages, while for BR and RR, the genetic background was more important in forming groups. The differential expression of proteins could be associated with fruit morphology traits such as a 72 kDa polypeptide present in MG stage with fruit diameter, height and mass and a 47 kDa polypeptide found in BR with fruit shelf life.