A 54 kDa polypeptide identified by 2D-PAGE and bulked segregant analysis underlies differences for pH values in tomato fruit

PEREIRA DA COSTA, JAVIER H; VEGA, TATIANA ALEJANDRA; PRATTA, GUILLERMO RAUL; PICARDI, LILIANA AMELIA; ZORZOLI, ROXANA; RODRÍGUEZ, GUSTAVO RUBÉN

ACTA PHYSIOLOGIAE PLANTARUM

SPRINGER HEIDELBERG

Lugar: HEIDELBERG; Año: 2017 vol. 39

0137-5881

Fruit pH is an important quality attribute in tomato and it isdefined during ripening. The aims of this work were to detect pericarppolypeptides associated with pH in an interspecific tomato BC1generation by 1D-PAGE and to identify those differentially expressedpolypeptides by comparing 2D-PAGE protein profiles from Bulked SegregantAnalysis (BSA). Polypeptide patterns were resolved by 1D-PAGE in a BC1population obtained by crossing the cv. ?Caimanta? of Solanum lycopersicum (recurrent parental genotype) and theaccession LA722 of S. pimpinellifolium(donor parental genotype). Putative QTL for fruit quality were detected bysingle point analysis. The presence of a 54-kDa band at the Mature Green stage(MG) carried by the wild genotype decreased the mean value of the pH trait. ABSA combined with 2D-PAGE was applied to the extreme phenotypes for pH in theBC1 segregating population. Four differentially expressed spots weredetected when the polypeptide patterns of the bulks were compared. The spotshad the expected molecular mass (around 54-kDa), and they were present in the lower-pHbulk and absent in the higher-pH one. The spots were identified by MS MALDI-TOFand two of them showed homology with the ATP synthase CF1 alphasubunit of Solanum lycopersicum. Theseresults indicate that the association between the polypeptide marker and afruit quantitative trait detected by 1D-PAGE would not only indicate geneticlinkage but could also be directly related with the gene underlying thequantitative trait.