Genetic Analysis of Tomato Fruit Ripening at Polypeptide Profiles Level through Quantitative and Multivariate Approaches

MARCHIONNI BASTE, EZEQUIEL; PEREIRA DA COSTA, JAVIER H; RODRÍGUEZ, GUSTAVO RUBÉN; ZORZOLI, ROXANA; PRATTA, GUILLERMO RAUL

AMERICAN JOURNAL OF PLANT SCIENCES

SCIENTIFIC RESEARCH PUBLISHING

Lugar: Amsterdam; Año: 2014 vol. 5 p. 1926 - 1935

Multivariate analysis became essential in functional and structural Genomics because of the largequantity of biological data provided by these new research areas. Diallel mating design was widelyapplied to analyze the heritability of quantitative traits but it was recently used for approaching tothe inheritance patterns of other levels of gene expression such as transcript profiles. Investigatingthe inheritance pattern of total polypeptide profiles with a diallel design remains as a vacancysubject. The objective of the present research was to infer the inheritance of total polypeptidesprofiles from tomato pericarp tissue at four different ripening stages in a diallel mating design including five recombinant inbred lines (RILs) and their ten second cycle hybrids (SCH). To achieve this objective, a multivariate analysis was applied to identify eventual inheritance patterns through a data mining approach and then univariate analyses were used to verify these patterns.Mainly dominance and also overdominance, though in a minor percentage, contributed to the geneactions involved in their genetic basis. Multivariate analysis was efficient in identifying inheritancepatterns of total polypeptide profiles through a data mining approach, and univariate analyseslargely verified the identified gene actions.