Desmoglein-4 deficiency increases resident CD45+ leukocytes population in skin.

PIETROBON EO; MORENO-SOSA MT; PENNACCHIO GE; SOAJE M; VALDEZ SR; JAHN GA; MACKERN OBERTI JP

Mendoza

Congreso; XXXIV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2016

Sociedad de Biología de Cuyo

Desmogleins are involved in cell to cell adhesion mechanisms and are crucial in keeping structural integrity of different tissues including skin and heart. These family of molecules, e.g. desmoglein-3, modulate keratinocyte activation and may control key molecules such as actin and p38 MAPK. However, whether desmoglein-4 may drive inhibitory or activating signals to skin-resident immune cells is unknown. The aim of our work was to assess the impact of desmoglein-4 deficiency in the amount of skin leukocyte populations. To this end, OFA hr/hr rats (n=3) which are mutant for the desmoglein-4 gene and their strain of origin, Sprague-Dawley (SD) (n=3) rats were used in this study. Skin biopsies from OFA and SD rats were weighed, minced to obtain cell suspensions and stained with monoclonal antibodies against CD45 (pan-leukocyte marker) and CD3 (T cell marker), conjugated with APC and FITC respectively. Beads were added to stained cell suspensions from skin biopsies derived from OFA and SD rats and acquired by FACS to measure total cell counts per milligram of tissue. OFA rats showed an expansion of CD45+ cells compared to SD control rats (SD 1,2±0,1 vs OFA 3,9±0,5; cells/mg) (t test; p