INVESTIGADORES
JAHN Graciela Alma
congresos y reuniones científicas
Título:
Hypothyroidism alters PI3K/AKT signaling during lactation.
Autor/es:
CAMPO VERDE ARBOCCO F; PERSIA FA; HAPON MB; CARON RW; JAHN GA
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017
Institución organizadora:
Reunión Conjunta de Sociedades de Biociencias
Resumen:
Lactation is key in mammary differentiation and is regulated by prolactin and the JAK2/STAT5 and AKT signaling pathways. It is known that hypothyroidism (hypoT) has deleterious effects on the JAK2/STAT5 pathway. In this work, we evaluated the impact of PTU-induced hypoT on lactating rat mammary AKT pathway, which has been intimately linked with mammary cellular differentiation, transcriptional and translational cell performance and mammary pathologies like cancer. We evaluated the expression level and phosphorylation of the three AKT isoforms and the two PI3K sub-units by western blot in the mammary glands (n = 6) from rats on lactation days 2, 7 and 14 (L2, L7 and L14). Results were analyzed by twoway ANOVA and Bonferroni post-test. We found that on L7 hypoT decreased the expression level of the p85 subunit of PI3K (p