INVESTIGADORES
JAHN Graciela Alma
congresos y reuniones científicas
Título:
Role of estradiol in animals prenatally exposed to amphetamine on stress response and dopamine receptors in the nucleus accumbens.
Autor/es:
PENNACCHIO GE; NEIRA FJ; PIETROBON EO; JAHN GA; VALDEZ SR; BREGONZIO C; SOAJE M
Lugar:
Mar del lata
Reunión:
Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica,; 2016
Resumen:
The psychostimulants activate the same neurobiological substrates than stress response. Prenatal exposure to amphetamine (PEA) induces endurable changes that become manifest in the adulthood. These changes can be expressed as an altered central dopaminergic activity. Sex steroids modulate brain dopaminergic systems and may influence the neuroadaptive changes induced by amphetamine. Stress exposure induces dopamine release in the mesolimbic pathway and the activation of hypothalamic-pituitaryadrenal axis. Our aim was to evaluate the effect of estradiol in PEA animals in response to stress and dopaminergic receptors and tyrosine hydroxylase (TH) expression in nucleus accumbens. To this purpose, female rats were treated daily with amphetamine 2,5mg/ kg i.p or saline during days 15 to 21 of pregnancy. Their offspring were ovariectomized (OVX) at day 60, treated with estrogen (E2) (2 x 5ug/rat/24 h) or oil 15 days later, and subjected to immobilization stress during 2 h. Blood and tissue samples were collected to determine corticosterone by RIA and dopamine receptors (D1R, D2R, D3R) and TH expression by real time PCR in extracts of nucleus accumbens. Stress exposure increased corticosterone levels in OVX (basal: 109,19 ±44,57 ng/ml vs stress: 214,41±21,49 ng/ml) and OVX+E2 (basal: 157,24±33,87 ng/ml vs stress: 364,04±23,75 ng/ml), in both groups PEA blunted the corticosterone response to stress. PEA induced a decrease in basal D3R expression and an increase in TH expression in response to stress, both responses were E2 dependent. No change was observed in D1R and D2R expression. We conclude that the neuroadaptive response induced by PEA in nucleus accumbens is under estrogen influence. Moreover, an altered stress response should be considered between the neuroadaptive changes induced by PEA.