Effect of ovariectomy on PRL and hypothalamic expression of tyrosine hydroxylase (TH), receptors and PRL signaling molecules on lactating rats.

CAMPO VERDE ARBOCCO F; BONAFEDE MM; VALDEZ SR; SOAJE M; JAHN GA

Mendoza - Argentina

Congreso; XXVIII Reunión Científica de la Sociedad de Biología de Cuyo,; 2010

Sociedad de Biología de Cuyo

Ovariectomy (OVX) does not impair suckling induced PRL release although ovarian steroids are key players in the hypothalamic regulation of PRL secretion by TIDA neurons. To investigate the effect of OVX on days 2 (L2) or 10 (L10) of lactation on ovarian hormone receptors and PRL signaling mechanisms we determined by real time PCR, the hypothalamic expression of tyrosine hydroxylase (TH). E2 (ER), PRL (PRLR) and P4 (PR) receptors, STAT5b (PRL signaling mediator), SOCS3 and CIS (PRL signaling suppressors) on 12-day lactating rats. OVX on L2 or L10 had similar effects, lowering the expression of PRLR (Control 235±29 p