INVESTIGADORES
JAHN Graciela Alma
congresos y reuniones científicas
Título:
Expression of members of the prolactin signaling pathway in rat hypothalamus and regulation by thyroid hormones on late pregnancy.
Autor/es:
PENNACCHIO GE; NAVAS P; SOAJE M; CARREÑO NB; JAHN GA; VALDEZ SR
Lugar:
Mendoza - Argentina
Reunión:
Congreso; XXVIII Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2010
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
Thyroid disorders compromise fertility in women in reproductive age and cause pregnancy disorders and lactation failure. Hyperthyroidism alters prolactin (PRL) secretion at the end of pregnancy, advances delivery and impairs lactation in rats. Using real time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) we explored the changes in mRNA expression of long PRL receptor (PRLR), STAT5b (required for the negative-feedback action of PRL on hypothalamic dopaminergic neurons), SOCS-1, SOCS-3, CIS (proteins that disrupt downstream STAT translocation to the nucleus to suppress prolactin signaling) in medial basal hypothalamus (MBH) during late pregnancy in hyperthyroid and normal rats. Thyroxin-treated (HT, 250 µg/kg/day) or vehicle-treated (Co) rats were mated 8 days after the start of treatment and killed at days 20 (G20) and 21 (G21) of pregnancy. Serum concentrations of thyroid hormones increased in HT rats. HT increased MBH PRLR on G21 (1.01±0.12 vs Co 0.62±0.12, p