INVESTIGADORES
CHAN Raquel Lia
artículos
Título:
A sunflower WRKY transcription factor stimulates the mobilization of seed stored reserves during germination and post-germination growth
Autor/es:
RAINERI J; HARMAN MD; CHAN RL; IGLESIAS AA; RIBICHICH KF
Revista:
PLANT CELL REPORTS
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2016
ISSN:
0721-7714
Resumen:
Germinating oilseeds convert stored lipids into sugarsand thereafter in metabolic energy that is used in seedling growth and establishment.During germination, the induced lipolysis linked to the glyoxylate pathway andgluconeogenesis produces sucrose which is then transported to the embryo, whereit is driven through catabolic routes. Herein, we report that the sunflowertranscription factor HaWRKY10 regulates carbon partitioning by reducing carbohydratecatabolism and increasing lipolysis and gluconeogenesis. HaWRKY10 was regulated by ABAand gibberelins in the embryo leaves and highly expressed during sunflower seedgermination and seedling growth, concomitantly with lipid mobilization.Sunflower leaf discs overexpressing HaWRKY10showed as repressed the expression ofgenes related with sucrose cleavage and glycolysis compared to controls. Moreover,HaWRKY10 constitutive expression inArabidopsis seeds produced higher decrease in lipid reserves, whereas starchand sucrose were more preserved compared to wild type. Gene transcriptsabundance and enzyme activities involved in stored lipid mobilization and gluconeogenesisincreased more in transgenic than in wild type seeds 36 hours after imbibitionwhereas the negative regulator of lipid mobilization, ABI4, was repressed. Altogether, the results point out a functionalparallelism between tissues and plant species, and reveal HaWRKY10 as apositive regulator of storage reserve mobilization in sunflower.