Protection of Chimerica subcellular vaccines against Brucella ovis in rams.

ESTEIN SM; FIORENTINO MA; PAOLICCHI FA; CLAUSE M; MANAZZA J; JULIANA CASSATARO; GIAMBARTOLOMEI GH; CORIA LM; FOSSATI CA; GOLDBAUM FA

Tandil

Congreso; XV Reunión Anual de La Sociedad Argentina de Farmacología Experimental (SAFE).; 2008

<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} @page Section1 {size:595.3pt 841.9pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:35.4pt; mso-footer-margin:35.4pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Chimera BLS-OMP31 as a recombinant protein (BLS-Omp31) or DNA vaccine (pCIbls-Omp31) have been identified as protective antigens against B. ovis in mice. Groups of 10 rams were vaccinated three times with: a) Chimera in oil based adjuvant (AFI), b) Chimera in saponin (QUIL A), c) DNA with electro-poration, d)  DNA without electroporation and e) Prime-boost (PB) (3 times with electroporated DNA, and a fourth with protein). Control group was immunized with hot saline extract (HS) of B. ovis. Rams were challenged with virulent B. ovis 7 months after last immunization and slaughtered 6 months thereafter, taking bacteriological samples from 12 organs. Chimera in AFI and PB  strategy induced the highest IgG specific antibodies followed by chimera in  QUIL A. Electroporation enhanced humoral immune response in DNA vaccinated rams. However PB stimulated the best levels of specific gamma IFN. The highest rates of protection were obtained with PB (75%) and chimera with AFI (63%). In the other groups the level of protection reminded between 10-20 %, including HS vaccine. Percentages of infection in unvaccinated rams and  DNA without electroporation were 100 %. Chimera should be considered as potential vaccine in ovine brucellosis.