Immunomodulatory effect of U-Omp19 in a papain-induced allergy model.

CASTRO, CELESTE PUEBLAS; CORIA, LORENA; BRUNO LAURA; PASQUEVICH KARINA; CASSATARO JULIANA

Buenos Aires

Congreso; Reunión Anual de Sociedades de Biociencia SAIC ? SAI ? SAFIS; 2020

SAIC ? SAI ? SAFIS

Allergies are T helper 2 immune responses mediated by IgE, induced by an antigen called allergen. Many allergens are proteases; thus, allergy models were developed using cysteine proteases like papain.At our laboratory we had demonstrated the potential of U-Omp19 as an adjuvant in vaccine formulations against infectious diseases inducing a Th1 response. Therefore, the main objective of this work was to evaluate its potential to modulate allergic responses. To this end an allergy model induced by papain was performed at our laboratory. Mice (n=5/group) were subcutaneously immunized with OVA, OVA+papain or OVA+papain+U-Omp19. The antibody response was assessed by analyzing anti-OVA IgE, IgG1 and IgG2a in serum by indirect ELISA. The specific cellular immune response was also measured, analyzing the levels of cytokines associated with a Th2 response (IL-5 and IL-13) in OVA-stimulated splenocyte supernatants by ELISA.Our results indicate that the allergic response was induced when mice were immunized with OVA plus papain. In this group, we have found a significant increase in anti-OVA IgE levels (p