INVESTIGADORES
AYBAR Manuel Javier
congresos y reuniones científicas
Título:
Effect of temperature on growth and lipid production of the cold-adapted Rhodotorula glutinis strains
Autor/es:
M. VIRGINIA ANGELICOLA; SILVANA C. VIÑARTA; MANUEL J. AYBAR; LUCIA I. CASTELLANOS FIGUEROA
Lugar:
Bariloche
Reunión:
Simposio; XXXIV International Specialized Symposium on Yeast; 2018
Institución organizadora:
ISSYeast
Resumen:
Cold-adaptedoleaginous yeasts are excellent lipid-producers and they are considered as apotential source of triglycerides (TAG) for the biodiesel synthesis. Thelipogenesis is influenced by temperature. This work evaluated the effect ofdifferent temperaturas on the growth and lipids accumulation by two strains of Rhodotorulaglutinis (R4 and R48). Yeasts were cultured (168 h, 250 rpm) at 15, 20 and25°C in nitrogen-limited glucose-based medium (MI). An increase of biomass,lipid production and total lipids content was observed along the culture timefor the yeasts at the three incubation temperatures. The highest biomass(11.48-13.53 g L-1) was yielded after 72 h at 20 and 25°C andremained constant until the end of the culture. At 15°C the growth was slower forboth strains. Effects of temperature on lipid production and accumulation weremore pronounced in R48 strain. The highest values of these parameters werereached at 20° C (5.64 g L-1; 45.74) and 25°C (5.48 g L-1; 49.15%) after 120 h of culture. While at 15 °C results werelower. R4 was less affected by temperature and significant differences were notobserved. The higher lipid production (5.68-7.00 g L-1) and lipids accumulation (48.02-53.00%) were yielded after 120 h.The presence of TAG in microbial lipids was determined qualitatively using thinlayer chromatography (TLC). The results showed that these yeasts are able tosynthesize TAG under the culture conditions tested. Qualitatively, there was anincrease in the production of TAG (larger size and density of spots) withtemperature. The fatty acids profile determined by GC-FID is suitable for biodieselsynthesis. R4 and R48 are interesting yeasts for microbial oils production and theyare capable to grow and produce lipids between 15 and 25°C.