INVESTIGADORES
AYBAR Manuel Javier
congresos y reuniones científicas
Título:
Edn1/Ednra pathway in Xenopus neural crest development
Autor/es:
MARCELA BONANO; CELESTE TRÍBULO; JAIME DE CALISTO; ROBERTO MAYOR; SARA S. SÁNCHEZ; MANUEL J. AYBAR
Lugar:
Ann Arbor, Michigan, USA
Reunión:
Congreso; 65th Annual Meeting of the Society for Developmental Biology; 2006
Institución organizadora:
Society for Developmental Biology
Resumen:
The neural crest (NC) develops at the border between neural plate and the prospective epidermis in all vertebrate embryos. NC cells are highly migratory and generate a number of derivatives including neurons, pigment cells, smooth muscle, craniofacial cartilage, endocrine cells, etc. Numerous studies have demonstrated that the signals BMP4, Wnt, FGF, Notch/Delta and retinoic acid are involved in the initial induction of this tissue. However, the participation in neural crest specification of other cell signaling pathways has not been established. In this work, we have analyzed the expression and participation of the Endothelin1/Endothelin Receptor A cell signaling pathway during the development of Xenopus laevis. We report the isolation of the cDNA of Ednra, and a detailed analysis of its expression pattern during development. Results showed that Ednra is expressed at the neural plate border from early neurula stage, in the neural crest during migration, and in branchial arches and the otic vesicle. The participation of Edn1/Ednra pathway during development was analyzed by conditional gain of function through the overexpression of Ednra mRNA, while the loss of function approach was performed using a specific inhibitor of Ednra named BQ123, and by the microinjection of specific Ednra morpholino antisense oligonucleotides. We also present embryological experimental evidence showing Edn1/Edrna pathway is involved in the maintenance of the specification and in cell survival of the neural crest. Our results suggest that Edn1/Ednra signaling pathway is required for the initial induction and migration of neural crest cells in Xenopus embryos.