INVESTIGADORES
FELICE Carmelo Jose
artículos
Título:
Automatic on-line analyzer of microbial growth using simultaneous measurements of impedance and turbidity
Autor/es:
MADRID, ROSSANA ELENA; FELICE, CARMELO JOSÉ; VALENTINUZZI, MÁXIMO EUGENIO
Revista:
Medical & Biological Engineering and Computing
Referencias:
Año: 1999 vol. 37 p. 1 - 5
Resumen:
An apparatus for the measurement of bacterial growth is described. The instrumentapplies alternate adequate sequential currents of two different frequencies through a pair ofelectrodes immersed in a cultured medium. It monitors, detects and quantifies the growth ofmicroorganisms based on the measurement of the impedance across the two electrodes and,simultaneously, it measures the variation of the medium turbidity. The medium conductivityand the interface electrode impedance changes can be dissected out from the measuredimpedance. The variations of turbidity can be calibrated in absorbance or optical densityunits. Moreover, all these parameters are also proportional to bacterial proliferation. Thiscomputer-controlled apparatus processes and displays them on a monitor showing bulkresistance, electrode impedance and turbidity changes as time course events. The equipmentcan detect aerobic or anaerobic microorganisms and permits the operator to simultaneouslyassess impedance and turbidity, or it can produce each parameter as a separate event. Timegrowth curves of different microorganisms are presented in the results.